In the video, the narrarator says:
"Generally, their faces appear to be 15 to 20 years younger than their counterparts in other areas of the world."
Years ago, I saw an aesthetician who told me the reason her skin is so clear and beautiful is because she takes lots of pycnogenol - which was the strongest antioxidant I'd ever heard of, before I learned about the antioxidant water I'm a huuge fan of. After experimenting with different antioxidants for the last 10 years, I have to say, I have seen for myself that antioxidants really do make a difference in your skin. I have a liver spot on my face (which my sister and mom also have in the same spot) and I can tell, when I'm taking antioxidants, it fades. When I'm not, it gets darker!
Anyway, I did a little research on this subject, and sure enough, I found this study below. You can see the original study on this page:
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400006
I've highlighted the part of the study that says:
The current study revealed significant antimicrobial, particularly antibacterial, activity of the extracts of both sweet and bitter apricot kernels. Additionally, high antioxidant activity was recorded, especially in sweet kernel extracts.
I have a theory that antioxidants are BENEFICIAL for patients who have cancer. Doctors will try to tell you the opposite. Please have a look at what Dr. Gonzalez has to say about antioxidants and chemotherpy.
Here is another video, featuring Harvard graduated surgeon, Horst Filtzer, on how antioxidants are beneficial, and not proven to negate the effects of chemotherapy. The water he's talking about is the same type of water make at home (same machine, same brand). You can learn more about that, HERE. I am absolutely NOT saying it cures or treats cancer. What I am saying is, it appears that antioxidants are beneficial for cancer, and this water is the cheapest, fastest, most effective way that I know of, to get a massive amount of antioxidants into the body.
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PermalinkBrazilian Journal of Medical and Biological Research
Print version ISSN 0100-879XOn-line version ISSN 1414-431X
Braz J Med Biol Res vol.42 no.4 Ribeirão Preto Apr. 2009
http://dx.doi.org/10.1590/S0100-879X2009000400006
Antioxidant and antimicrobial activities of bitter and sweet apricot (Prunus armeniacaL.) kernels
D. Yiğit1, N. Yiğit2 and A. Mavi3
1Department of Science Education, Education Faculty, Erzincan University, Erzincan, Turkey
2Health Services Vocational Training School, Medical Laboratory Department, Atatürk University, Erzurun, Turkey
3Kazim Karabekir Education Faculty, Atatürk University, Erzurum, Turkey
2Health Services Vocational Training School, Medical Laboratory Department, Atatürk University, Erzurun, Turkey
3Kazim Karabekir Education Faculty, Atatürk University, Erzurum, Turkey
Abstract Introduction Material and Methods Results and Discussion References Correspondence and Footnotes
The present study describes the in vitro antimicrobial and antioxidant activity of methanol and water extracts of sweet and bitter apricot (Prunus armeniaca L.) kernels. The antioxidant properties of apricot kernels were evaluated by determining radical scavenging power, lipid peroxidation inhibition activity and total phenol content measured with a DPPH test, the thiocyanate method and the Folin method, respectively. In contrast to extracts of the bitter kernels, both the water and methanol extracts of sweet kernels have antioxidant potential. The highest percent inhibition of lipid peroxidation (69%) and total phenolic content (7.9 ± 0.2 µg/mL) were detected in the methanol extract of sweet kernels (Hasanbey) and in the water extract of the same cultivar, respectively. The antimicrobial activities of the above extracts were also tested against human pathogenic microorganisms using a disc-diffusion method, and the minimal inhibitory concentration (MIC) values of each active extract were determined. The most effective antibacterial activity was observed in the methanol and water extracts of bitter kernels and in the methanol extract of sweet kernels against the Gram-positive bacteria Staphylococcus aureus. Additionally, the methanol extracts of the bitter kernels were very potent against the Gram-negative bacteria Escherichia coli (0.312 mg/mL MIC value). Significant anti-candida activity was also observed with the methanol extract of bitter apricot kernels against Candida albicans, consisting of a 14 mm in diameter of inhibition zone and a 0.625 mg/mL MIC value.
Key words: Apricot (Prunus armeniaca L.) kernels; Antioxidant activity; Antimicrobial activity; Total phenolic compounds
In the past few years, there has been a renewed interest in evaluating the antioxidant content and distribution patterns of fruits and vegetables. This change occurred after it was discovered that antioxidant phytonutrients (carotenoid and phenolics) are important for two main reasons. First, they are responsible for the sensory properties of food and second, they have protective activity against a variety of degenerative diseases (1,2).
Currently, there is overwhelming evidence indicating that free radicals cause oxidative damage to lipids, proteins and nucleic acids. Free radicals may lie at the heart of the etiology or of the natural history of a number of diseases, including cancer and atherosclerosis (3-5). Therefore, antioxidants, which can neutralize free radicals, may be of central importance in the prevention of these diseases (6).
Fruits and vegetables contain many different antioxidant components. For example, some flavonoids (including flavones, isoflavones, flavonones, anthocyanins, catechin, and isocatechin) that are frequent components of the human diet have demonstrated strong antioxidant activities (7). In fact, higher plants exhibit significant potency against human bacterial and fungal pathogens. Apart from being the primary food source of some essential nutrients, fruits and vegetables also contain a variety of bioactive components, which might have other beneficial health effects (8).
The apricot (Prunus armeniaca L.) is a member of the Rosaceae family. Apricot fruit, being a rich source of vitamins and minerals, is one of the most familiar crops worldwide. Apricot trees are not ubiquitous since they can only grow in certain regions where the environmental conditions are favorable (9). The apricot is native to China and Japan, but is also cultivated in the warmer temperate regions of the world, including Turkey, through southern Europe, South Africa and Australia. In Turkey, the apricot is grown mainly in Malatya and Erzincan where the climate permits economic production (10).
The apricot has been used in folk medicine as a remedy for various diseases. A decoction of the plant bark has functioned as an astringent to soothe irritated skin. Other uses for apricots in folk medicine include treatment of hemorrhages, infertility, eye inflammation, and spasm. Apricot kernel paste can heal vaginal infections. The kernel oil has been used in cosmetics and as a pharmaceutical agent (laxative and expectorant). In very small amounts, the toxic hydrogen cyanide present in bitter apricot kernels has been prescribed for asthma, cough and constipation (11).
The fresh apricot fruit contains carbohydrates, vitamins C and K, β-carotene, niacin, and thiamine. Organic acids, phenols, volatile compounds, esters, and terpenoids have also been isolated (2,12-14).
Apricot kernels contain a substantial amount of dietary protein (15), along with significant amounts of oil and fiber (16,17). In a previous study, Femenia et al. (18) reported that sweet apricot kernels contain more oil than bitter kernels, and that oleic acid and linoleic acid correspond to approximately 92 g/100 g of the total fatty acids present.
Apricot kernels, depending on the variety, contain the toxic cyanogenic glycoside amygdalin (19). Amygdalin can be hydrolyzed to form glucose, benzaldehyde and hydrocyanic acid. Enzymatic release of cyanide occurs in the presence of β-glucoronidase, an enzyme found in the human intestine (20).
Numerous reports concerning the physicochemical characteristics of apricot seed oils are available in the literature (2,21,22), but little information is available concerning the in vitro antioxidant activities of these oils (23). Similarly, the antimicrobial activities of apricot kernels have been poorly studied (24), and the lack of studies concerning the antibacterial and antifungal activity of the kernels have stimulated the present research.
Thus, the objective of the present study was to determine the antioxidant and antimicrobial activities of methanol and water extracts of both bitter and sweet kernels of P. armeniaca L.
Preparation of extracts
Apricot fruits (P. armeniaca L.) were collected at their optimum commercial maturity when enologically ripe in Uzumlu Town, Erzincan, Turkey. Bitter kernels belong to wild apricot fruits locally known as Zerdali, and sweet kernels are locally known as Hasanbey. The fresh fruit samples were transported to the laboratory where the stones were removed, and individual stones were hammered to obtain the seed kernel. Next, the skin was removed, and the kernels were dried on the bench and then ground to a powder using an electric blender. Powdered kernels were extracted with methanol in a Soxhlet apparatus for 24 h. Subsequently, the methanol was evaporated with a rotary evaporator. After evaporation of the solvent, the w/w yield from the dry starting material was 14.3 and 12.6% for Hasanbey and Zerdali kernels, respectively. Water extracts were also prepared by adding 20 g powdered material to 200 mL boiling water in a glass flask, and the solutions were incubated at room temperature for 2 h on a rotating shaker (200 rpm). The mixture was filtered through Whatman (No. 1) filter paper, and the filtrate was lyophilized. The kernel extract yields were 8.2 (w/w) and 7.9% (w/w) for the Hasanbey and Zerdali cultivars, respectively. All extracts were stored in a freezer at _24°C until use.
Antimicrobial activity
Tests were carried out on clinical isolates of 96 bacterial strains belonging to five bacterial species (Enterobacter aerogenes, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Staphylococcus aureus) and 91 Candida strains belonging to nine yeast species (Candida albicans, C. glabrata, C. guilliermondii, C. kefyr, C. krusei, C. parapisilosis, C. pseudotropicalis, C. tropicalis, and Geotrichum candidum). Microorganisms were provided by the Department of Clinical Microbiology, Faculty of Medicine, Erzurum. The Candida isolates were identified using standard taxonomic procedures, including analysis of germ tube production, microscopic appearance on cornmeal agar with Tween-80, the production of chlamydospores, colony morphology, and pigment production on chromogenic medium. The identification was confirmed by the API 32C AUX (BioMeriux, Marcy-l'Etoile, France) identification system for yeast.
The Gram-negative bacteria were submitted to tests including the indol test, motility, methyl red, hydrogen sulfide production, urease and phenylalanine decarboxylase production, growth in Simmons citrate and fermentation of glucose, lactose, saccharose, and mannitol. The colonies with a Gram-positive coccus morphology isolated in blood agar were submitted to tests of colony morphology, pigment production, Gram stain, and catalase and coagulase production.
Disc-diffusion assay. The dried methanol and water extracts were dissolved in the extraction solvent (methanol or sterile distilled water) to a final concentration of 30 mg/mL. Antimicrobial tests were than carried out using a disc-diffusion method (25) with a suspension containing 108 colony forming units/mL of bacteria and 106 colony forming units/mL of yeast spread on nutrient agar (Oxoid, USA). Extracts were applied to the discs (6 mm in diameter) and allowed to soak in, and were then placed on the inoculated nutrient agar. Negative controls were prepared using the same solvents as employed to obtain the extracts. As positive controls, ofloxacin (5 µg, Oxoid) was used for Gram-positive bacteria, cefaperazone_sulbactam (105 µg, Oxoid) for Gram-negative bacteria and amphotericin B (30 µg, Sigma) for Candida spp. The inoculated plates were incubated at 37°C for 24 h for clinical bacterial strains and at 35°C for 48 h for yeast. Antimicrobial activity was evaluated by measuring the inhibition zone against test microorganisms.
The minimal inhibitory concentration (MIC) was also determined for each microorganism that was sensitive to the methanol and/or water extracts in the disc-diffusion assay. MIC values for extracts against microbial strains were determined based on a micro-well dilution method (26). The inoculations of microorganisms were prepared from 12-h broth cultures, and suspensions were adjusted to 0.5 McFarland standard turbidity. First, the extracts were dissolved in 10% dimethyl sulfoxide in water to a dilution of 5 mg/mL, and serial 2-fold dilutions were prepared in a sterile test tube containing nutrient broth for a specific range of concentrations (0.078-5 mg/mL). The 96-well plates were prepared by dispensing 95 µL nutrient broth and 5 µL of the inocula into each well. A volume of 100 µL from each extract prepared at the highest concentration was added to the first well, and then 100 µL from each serial dilution was transferred to other consecutive wells. The plates were covered with a sterile plate sealer and then incubated for 24 h (for bacterial strains) and 48 h (for fungal strains). The MIC was defined as the lowest concentration of the extract that would inhibit the growth of the microorganism.
Antioxidant activity
Antioxidant activity was determined by the thiocyanate method (27). Briefly, stock extract solutions were prepared at 2 mg/mL concentration of required stock solutions and mixed with 2.5 mL 20 mM linoleic acid emulsion (Fluka, Germany) containing an equal weight of Tween-20 (Sigma, USA) in phosphate-buffered saline, pH 7.4 (Sigma). The final volume was adjusted to 5 mL with 20 mM phosphate-buffered saline, pH 7.4, in a test tube and incubated in the dark at 40°C. The final concentration of the extracts was 100 µg/mL. Butylated hydroxytoluene (Sigma) was used as positive control (100 µg/mL). The amount of peroxide was determined by measuring absorbance at 500 nm after coloring yellow with FeCl2 and thiocyanate during a 24-h incubation. Lower absorbance indicates higher antioxidant activity. To eliminate the solvent effect, the same amount of solvent that was used to prepare the solutions of test samples was added to the control test sample, which contained the linoleic acid emulsion. Measurements of antioxidant activity were carried out in three experimental replicates, and values are reported as the average of three replicates. This activity is reported as percent lipid peroxidation compared to the solvent control.
DPPH radical-scavenging activity
Experiments were performed as described previously (28). Briefly, a 0.5 mM 2,2-diphenyl-1-picrylhydrazyl (DPPH; Fluka) radical solution was prepared in methanol, and 1 mL of this solution was mixed with 3 mL of the sample solution. Final concentrations of essential oils were 100 and 300 µg/mL. Butylated hydroxytoluene was used as a positive control at the same concentration. After incubation for 30 min in the dark, absorbance at 517 nm was measured. A decreasing absorbance of the DPPH solution indicates an increase in DPPH radical scavenging activity. This activity is reported as a percent of DPPH radical scavenging.
The control contained 1 mL DPPH solution mixed with 3 mL ethanol. DPPH radical scavenging activity was measured in duplicate, and the values are reported as the average.
Determination of total phenolic compounds
Antioxidant compounds generally contain phenolic group(s). Because of this, the amounts of phenolic compounds in each extract were compared. The analysis was performed as described previously (27). Aliquots of the extract were diluted to 4 mL in water, 0.25 mL Folin-Ciocalteu reagent (Fluka) was added and after 3 min 0.75 mL of a 2% Na2CO3 solution was added. Subsequently, the mixture was transferred to a shaker for 2 h at room temperature and absorbance at 760 nm was measured. The amount of total phenolic compounds was calculated for two different experiments, and the values reported are the average of two replicates. Gallic acid was used as the standard for a calibration curve. The phenolic compound content was reported as gallic acid equivalents.
Statistical analysis
Statistical analyses were performed using the SPSS 12.0 software. To identify correlations between lipid peroxide inhibition, radical scavenging activity and total phenolic content, the data were analyzed by bivariate correlation using the Pearson correlation test. Values of P < 0.05 were considered to be statistically significant.
Antioxidant activity
The highest percent inhibition of lipid peroxidation was 68.6%/100 µg solid in methanol extracts of the sweet kernel (Hasanbey). This was followed by the water extract from the same cultivar, which demonstrated 66.3% inhibition. The lowest activities were detected in the methanol and water extracts of a bitter kernel with 3.7 and 20.2% inhibition, respectively.
The DPPH radical scavenging activities of water and methanol extracts from apricot cultivars are shown in Figure 1. At a concentration of 100 µg/mL, the water and methanol extracts of the sweet kernel exhibited 89.9 and 87.7% scavenging activity, respectively. However, at a concentration of 300 µg/mL, the respective activities were 89.9 and 92.2%. There was no noticeable effect of extract concentration for these extracts. Unlike sweet kernel extracts, there was no detectable activity in the bitter kernel extracts at the concentrations studied (100-300 µg/mL).
In order to determine the amount of phenolic compounds present in the extracts, the absorbance of the extract solutions was measured after incubating the samples with the Folin-Ciocalteu reagent. Like radical-scavenging activities and percent lipid peroxidation inhibition, the contents of phenolic compounds of both methanol and water extracts of the bitter kernel were lower than sweet kernel extracts, each containing 100 µg/mL solid (Table 1). The highest phenolic content (7.9 ± 0.2 µg/mL) was detected in the water extract of a sweet apricot kernel, while the lowest phenolic content was 0.4 ± 0.1 µg/mL in the water extract of the bitter apricot kernel.
According to the Pearson correlation test, there was a statistically significant correlation between DPPH radical scavenging and total phenolic compounds (r = 0.975, P < 0.01), between peroxide inhibition and total phenolic compounds (r = 0.942, P < 0.05), and between lipid peroxide inhibition and DPPH radical scavenging (r = 0.979, P < 0.05).
Antimicrobial activities
Table 2 shows the antimicrobial activities of methanol and water extracts of bitter (Zerdali) and sweet (Hasanbey) apricot cultivar kernels against clinical isolates of human pathogenic bacteria and yeast. The microorganisms examined in the present study belong to five bacteria and nine yeast species. The antimicrobial potency was quantitatively assessed (Table 2), and each active extract was used to calculate the MIC (Table 3).
The water and methanol extracts of bitter and sweet kernels showed antimicrobial activity against clinical isolates of microorganisms from six species, with inhibition zones ranging from 8 to 15 mm (Table 2). The results confirmed that both sweet and bitter kernels of apricot are potentially rich sources of antimicrobial agents. However, both fruit kernel extracts exhibited similar characteristics in their activity against test microorganisms, with differences being observed for only two microorganisms, P. mirabilis and C. glabrata.
None of the extracts exhibited antimicrobial activity against eight of the fourteen microorganisms, Enterobacter aerogenes, P. aeruginosa, C. guilliermondii, C. kefyr, C. krusei, C. pseudotropicalis, C. tropicalis, and G. candidum.
The highest activity was obtained with both methanol and water extracts of bitter kernels, which showed broad-spectrum antimicrobial activity against Gram-positive, Gram-negative and Candida strains. Specifically, these extracts produced inhibition zones of 8-15 mm and MIC values of 0.312-2.5 mg/mL (Tables 2 and 3).
The higher activity of bitter kernels, with inhibition zones of more than 12 mm, was observed for both the methanol and water extracts against S. aureus and E. coli and in methanol extracts against C. albicans. However, the water extracts exhibited activity against P. mirabilis with a 12-mm inhibition zone and a 0.625 mg/mL MIC value. Methanol extracts of the bitter kernel had anticandidal activity against C. glabrata, with a 10-mm inhibition zone and a 1.25 mg/mL MIC value. However, none of the sweet kernel extracts had any antimicrobial activity against these microorganisms.
Similar to the bitter kernel apricot extracts, higher activity was detected in sweet kernel extracts against E. coli with 13-mm inhibition zones. Additionally, moderate activity of sweet kernels, with an inhibition zone of more than 7 mm, was documented against S. aureus, C. albicans and C. parapisilosis (Table 2).
The results obtained in the current study indicate that the candidal strains are resistant to both kernel extracts screened. Similarly, it has been previously reported that fungi are more resistant than bacteria to plant extracts (29-31).
The butanol extract from P. armeniaca fruits showed inhibitory activity on disease-causing Gram-negative bacteria (31.25-250 µg/mL MIC values) and Gram-positive bacteria (125-250 µg/mL MIC values). The most inhibited was Micrococcus luteus (31.25 mg/mL MIC value) (32). Different findings have been reported in another study, indicating that the essential oil of apricots had no antimicrobial activity against some bacteria and yeast (23). On the other hand, high antimicrobial activity (90% inhibition) from the methanol extracts of Japanese apricot has been reported against Helicobacter pylori (33).
The current study revealed significant antimicrobial, particularly antibacterial, activity of the extracts of both sweet and bitter apricot kernels. Additionally, high antioxidant activity was recorded, especially in sweet kernel extracts. Although the inhibition of lipid peroxidation and the DPPH radical scavenging activities of the sweet kernel extracts were markedly higher than those of bitter kernel extracts, there were no marked differences in the antimicrobial activities of the above extracts. Therefore, we suggest that there is no clear relationship between the antioxidant activities and the antimicrobial activities of the extracts. In addition, these results indicate that apricot seeds could not only serve as a rich source of food but may also be of importance in ethno-botanical studies due to their high antioxidant and antimicrobial activities.
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